Meet Inspiring Speakers and Experts at our 3000+ Global Conference Series Events with over 1000+ Conferences, 1000+ Symposiums
and 1000+ Workshops on Medical, Pharma, Engineering, Science, Technology and Business.

Explore and learn more about Conference Series : World's leading Event Organizer

Back

Kathleen Hefferon

Kathleen Hefferon

Cornell University, USA

Title: Production of a universal plant-based substrate system for cellulase activity assays

Biography

Biography: Kathleen Hefferon

Abstract

Cellulases and other cell wall degrading enzymes are currently being engineered with improved traits for application in the breakdown of lignocellulosic biomass. The majority of assays with these ‘designer’enzymes have been carried out using synthetic substrates such as crystalline bacterial micro cellulose (BMCC). The use of synthetic substrates may not reflect the actual action of these cellulases on real plants. In the following study, suspension cell walls from several plant species were examined as possible alternatives for synthetic cellulose substrates. The results suggest that isolated plant cell walls can be used to reproducibly assay for cellulase activity.